KMID : 0043320150380071369
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Archives of Pharmacal Research 2015 Volume.38 No. 7 p.1369 ~ p.1379
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Anti-inflammatory, antioxidant and anti-acetylcholinesterase activities of Bouvardia ternifolia: potential implications in Alzheimer¡¯s disease
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Garcia Morales Giovanni
Huerta Reyes Maira Gonzalez Cortazar Manases Zamilpa Alejandro Jimenez Ferrer Enrique Silva Garcia Raul Roman Ramos Ruben Aguilar Rojas Arturo
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Abstract
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Bouvardia ternifolia has been used medicinally to treat inflammation. In the present study, we investigate the anti-Alzheimer¡¯s potential effect of the hydroalcoholic extract of B. ternifolia through evaluation of anti-inflammatory and antioxidant activities, quantification of the percentage inhibition of acetylcholinesterase activity, protection effect against ¥â-amyloid fibrillar-induce neurotoxicity, and the identification of the main constituents. Our results show that B. ternifolia extract and ethyl acetate fraction induced anti-inflammatory effects by reducing inflammation by >70 %, while antioxidant test revealed significant IC50 values for flavonoid content fraction (30.67 ¡¾ 2.09 ¥ìg/ml) and ethyl acetate fraction (42.66 ¡¾ 0.93 ¥ìg/ml). The maximum inhibition of acetylcholinesterase was exhibited by scopoletin content fraction (38.43 ¡¾ 3.94 %), while ethyl acetate fraction exerted neuroprotective effect against ¥â-amyloid peptide (83.97 ¡¾ 5.03 %). Phytochemical analysis, showed the presence of 3-O-quercetin glucopyranoside (415 mg/g), rutin (229.9 mg/g), ursolic and oleanolic acid (54 and 20.8 mg/g respectively), 3-O-quercetin rhamnopyranoside (12.8 mg/g), chlorogenic acid (9.5 mg/g), and scopoletin (1.38 mg/g). Our findings support the use of B. ternifolia since the extract induced significant neuroprotection against ¥â-amyloid peptide, anti-inflammatory, antioxidant and anti-acetylcholinesterase effects that could be attributed to its contents of polyphenols, coumarins, and triterpenes, and encourage further studies for development of this extract as therapeutic agent in treatment of Alzheimer¡¯s disease.
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KEYWORD
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Bouvardia ternifolia, Alzheimer¡¯s disease, Polyphenols, Cumarins, Triterpens
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